Phospho-Ack1 (Tyr284) Antibody

• 产品编号：CST-3138S      品牌：CST       原厂货号：3138S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Ack1 (Tyr284) 抗体仅识别转染水平的Tyr284磷酸化的Ack1蛋白。通过合成与人源Ack1蛋白Tyr284邻近氨基酸残基序列一致的肽段，免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Ack1和Ack2 (activated cdc42-associated kinase 1 and 2)蛋白是非受体酪氨酸激酶，由一个酪氨酸激酶核心、一个SH3区域、一个Cdc42/Rac相互作用粘附区（CRIB）、一个 Ralt 同源区域和一个脯氨酸富足区域构成(1,2)。Ack1和Ack2是仅有的两个已知的与cdc42相互作用的酪氨酸激酶。通过生长因子包括EGF和PDGF以及细胞粘附分子包括活化的整合素可能激活两个Ack蛋白 ，Ack蛋白可能作用是联系酪氨酸激酶受体或与cdc42相关的G蛋白偶联受体信号。Acks 可能调节细胞生长、形态和动力(3,4)。最近研究发现指出Ack1蛋白可能在前列腺肿瘤的发生起着重要作用，这能使它对于这种类型癌症作为一个潜在药物靶点(5)。

1. Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
2. Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
3. Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
5. Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
6. Yokoyama, N. and Miller, W.T. (2006) Methods Enzymol. 406, 250-260.

原厂资料：

Specificity / Sensitivity

Phospho-Ack1 (Tyr284) Antibody detects transfected levels of Ack1 only when phosphorylated at Tyr284.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr284 of human Ack1. Antibodies are purified by peptide affinity chromatography.

Background

Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).

1. Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
2. Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
3. Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
5. Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
6. Yokoyama, N. and Miller, W.T. (2006) Methods Enzymol. 406, 250-260.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.