CDK6 (DCS83) Mouse mAb

• 产品编号：CST-3136S      品牌：CST       原厂货号：3136S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 CDK6 (DCS83) Mouse mAb 能够检测内源性CDK6总蛋白。该单克隆抗体是由重组人CDK6蛋白30-60位氨基酸免疫动物生产的。 周期暗白依赖性激酶和其蛋白伴侣和CDK抑制剂共同组成复合体。CDK6 和CDK4协同D-型周期蛋白并靶向Rb蛋白，促进G1/S期限制点的通过(1)。CDK6/cyclin D复合体的失活状态是游离的，通过结合INK4 CDK抑制剂家族的成员来实现(2,3)。与cdk抑制剂INK4家族不同，CDK抑制剂p21 Waf1/Cip1可以增强CDK4和CDK6及其与周期蛋白D伴侣的缔合(4)。

1. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
2. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
3. Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34.
4. Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9.

原厂资料：

Specificity / Sensitivity

CDK6 (DCS83) Mouse mAb detects endogenous levels of total CDK6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CDK6 corresponding to amino acids 30-65.

Background

The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of cdk inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).

1. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
2. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
3. Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34.
4. Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.