Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific)

• 产品编号：CST-3081S      品牌：CST       原厂货号：3081S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-C/EBPβ (Ser105)抗体（兔特异性）识别内源性的Ser105磷酸化的C/EBPβ。此抗体与其它磷酸化的C/EBP亚型α, δ, γ,ε或ζ没有交叉反应。它也不识别p20 LIP大鼠C/EBPβ亚型。该多克隆抗体用与大鼠C/EBPβ中Ser105位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。CCAAT/增强子结合蛋白（C/EBP）是一个转录因子家族，对细胞分化、终端功能和炎症反应十分关键（1）。目前已确定该家族的6个成员（C/EBPα, β, δ, γ, ε, ζ），它们分布在不同组织（1）。C/EBPβ有两种形式，38kDa的肝激活蛋白（LAP）和20kDa的肝抑制蛋白（LIP），它们可能是可变翻译的产物。38kDa LAP蛋白是转录激活因子，LIP是C/EBPβ的转录活性的抑制剂（2）。C/EBPβ不同位点的磷酸化刺激其转录活性（3-5）。大鼠C/EBPβ Ser105磷酸化是仅在大鼠序列中出现的一个独特位点，似乎对大鼠C/EBPβ的激活至关重要（6）。

1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
3. Wegner, M. et al. (1992) Science 256, 370-373.
4. Trautwein, C. et al. (1993) Nature 364, 544-547.
5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

原厂资料：

Specificity / Sensitivity

Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) detects endogenous levels of rat C/EBPbeta only when phosphorylated at serine 105. It does not cross-react with phosphorylated rat C/EBP family members alpha, delta, gamma, epsilon or zeta. It also does not recognize the p20 LIP rat C/EBPbeta isoform.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser105 of rat C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).

1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
3. Wegner, M. et al. (1992) Science 256, 370-373.
4. Trautwein, C. et al. (1993) Nature 364, 544-547.
5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.