Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb

• 产品编号：CST-3079S      品牌：CST       原厂货号：3079S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb能够检测内源性的苏氨酸(288位点)磷酸化的极光激酶A。该单克隆抗体是有合成的人源的针对极光激酶A苏氨酸(288位)磷酸化肽段免疫动物而生产的。极光激酶A(AIK)是一种调节细胞周期的丝氨酸/苏氨酸蛋白激酶，在多种肿瘤细胞系中过表达(1-3)。在蛋白激酶激活回路中，极光激酶A苏氨酸(288位)的磷酸化可显著提高其活性并靶向有丝分裂过程中蛋白质的蛋白酶体降解(4)。与极光激酶A密切相关的极光激酶B(AIM1)在多重有丝分裂事件中发挥作用，siRNA沉默其表达之后可以导致组蛋白H3磷酸化减少、异常的染色体排列/分离以及生存蛋白的定位改变(6)。

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

原厂资料：

Specificity / Sensitivity

Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb detects endogenous levels of Aurora A only when phosphorylated at Thr288.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr288 of human Aurora A.

Background

Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.