NEK7 (C34C3) Rabbit mAb

• 产品编号：CST-3057S      品牌：CST       原厂货号：3057S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NEK7 (C34C3) Rabbit mAb能够检测内源性NEK7总蛋白。该多克隆抗体是由合成的人源的针对NEK7蛋白羧基末端的肽段免疫动物生产的。人类NEK蛋白激酶家族由11个成员组成，它们含有一个共同的NIMA(一种鉴定自曲霉菌的丝氨酸/苏氨酸激酶)相关的氨基末端催化结构域。尽管真菌细胞周期进程中NIMA至关重要，哺乳动物细胞中NEK激酶的功能很大程度上是未知的。NEK1首次在小鼠cDNA表达文库筛选过程中被鉴定，具有双重的特异性激酶活性，基于酪氨酸和丝氨酸/苏氨酸两个位点(1)。NEK2主要和真菌NIMA的一级结构类似且被认为可以促进有丝分裂中复制中心体的分裂(2,3)。NEK3主要是细胞质酶，其活性在整个细胞周期呈边际变化(4)。NEK4分布广泛，其表达和亚细胞定位与细胞周期不相关(5)。NEK6/7被认为可以体外磷酸化并激活p70 S6激酶(6)。失活的NEK6突变体的表达能够在M期捕获细胞并干扰染色体分离(7)。NEK8的活性不参与细胞周期的调节，它可能在细胞周期非依赖的微管动力学中起作用(8)。NEK9在有丝分裂期被激活并能够参与NEK6/7的激活(9,10)。

1. Letwin, K. et al. (1992) EMBO J. 11, 3521-3531.
2. Fry, A.M. et al. (1995) J. Biol. Chem. 270, 12899-12905.
3. Fry, A.M. (2002) Oncogene 21, 6184-6194.
4. Tanaka, K. and Nigg, E.A. (1999) J. Biol. Chem. 274, 13491-13497.
5. Hayashi, K. et al. (1999) Biochem. Biophys. Res. Commun. 264, 449-456.
6. Belham, C. et al. (2001) Curr. Biol. 11, 1155-1167.
7. Yin, M.J. et al. (2003) J. Biol. Chem. 278, 52454-52460.
8. Holland, P.M. et al. (2002) J. Biol. Chem. 277, 16229-16240.
9. Belham, C. et al. (2003) J. Biol. Chem. 278, 34897-34909.
10. Roig, J. et al. (2002) Genes Dev. 16, 1640-1658.

原厂资料：

Specificity / Sensitivity

NEK7 (C34C3) Rabbit mAb detects endogenous levels of total NEK7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human NEK7.

Background

The NEK family of protein kinases is composed of 11 members in humans that share an amino-terminal catalytic domain related to NIMA, a serine/threonine kinase identified in Aspergillus nidulans. While NIMA is critical for cell cycle progression in fungus, the function of NEK kinases in mammalian cells is largely unknown. NEK1 was first identified by screening mouse cDNA expression libraries and was demonstrated to have dual specificity kinase activity on both tyrosine and serine/threonine sites (1). NEK2 most closely resembles fungal NIMA in its primary structure and is believed to promote the splitting of duplicated centrosomes at the onset of mitosis (2,3). NEK3 is predominantly a cytoplasmic enzyme and its activity shows marginal variation throughout the cell cycle (4). NEK4 is ubiquitously expressed and its expression and subcellular location are not associated with cell cycle (5). NEK6/7 have been suggested to phosphorylate and activate p70 S6 kinase in vitro (6). Expression of an inactive NEK6 mutant arrests cells in M phase and interferes with chromosome segregation (7). NEK8 activity is not cell cycle regulated and may play a role in cell cycle independent microtubule dynamics (8). NEK9 is activated during mitosis and may participate in the activation of NEK6/7 during mitosis (9,10).

1. Letwin, K. et al. (1992) EMBO J. 11, 3521-3531.
2. Fry, A.M. et al. (1995) J. Biol. Chem. 270, 12899-12905.
3. Fry, A.M. (2002) Oncogene 21, 6184-6194.
4. Tanaka, K. and Nigg, E.A. (1999) J. Biol. Chem. 274, 13491-13497.
5. Hayashi, K. et al. (1999) Biochem. Biophys. Res. Commun. 264, 449-456.
6. Belham, C. et al. (2001) Curr. Biol. 11, 1155-1167.
7. Yin, M.J. et al. (2003) J. Biol. Chem. 278, 52454-52460.
8. Holland, P.M. et al. (2002) J. Biol. Chem. 277, 16229-16240.
9. Belham, C. et al. (2003) J. Biol. Chem. 278, 34897-34909.
10. Roig, J. et al. (2002) Genes Dev. 16, 1640-1658.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.