NTF2 (5A3) Mouse mAb

• 产品编号：CST-3053S      品牌：CST       原厂货号：3053S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NTF2 (5A3) Mouse mAb检测内源性NTF2蛋白。通过重组人源全长的NTF2蛋白去免疫动物从而制备出单克隆抗体。小GTPase Ran定位于细胞质基质和核孔复合体（nuclear pore complex，NPC）的核膜面，并且调节不同蛋白进出和形成细胞核。像其它小GTPases一样，Ran蛋白存在GTP结合或GDP结合水平。RanGTP存在于细胞核同时能促进细胞核的输出功能，而细胞质的RanGDP促进输入。穿过核膜的RanGTP梯度允许货运蛋白的适当转移穿过NPC和有丝分裂纺锤体的维持(1-3)。Nuclear transport factor 2 (NTF2)调节Ran蛋白的亚细胞分布和功能(4-5)。通过与NPC蛋白的phenylalanine-glycine (FG)重复序列瞬间相互作用，NTF2 二聚体促进RanGDP的扩散穿过NPCs。以一个ATP依赖的方式通过细胞核因子，NTF2稳定Ran蛋白的GDP结合形式一直到它被诱导分离，从而允许鸟嘌呤核苷酸交换因子(GEF)RCC1 在Ran蛋白上去介导GDP交换成GTP(6-7)。

1. Mattaj, I.W. and Englmeier, L. (1998) Annu Rev Biochem 67, 265-306.
2. Kalab, P. et al. (2002) Science 295, 2452-6.
3. Becskei, A. and Mattaj, I.W. (2003) Proc Natl Acad Sci USA 100, 1717-22.
4. Ribbeck, K. et al. (1998) EMBO J 17, 6587-98.
5. Steggerda, S.M. et al. (2000) Mol Biol Cell 11, 703-19.
6. Stewart, M. (2000) Cell Struct Funct 25, 217-25.
7. Yamada, M. et al. (2004) J Biol Chem 279, 36228-34.

原厂资料：

Specificity / Sensitivity

NTF2 (5A3) Mouse mAb detects endogenous levels of total NTF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human NTF2.

Background

The small GTPase Ran resides on both the cytosolic and nucleosolic sides of the nuclear pore complex (NPC) and regulates the import and export of various proteins to and from the nucleus. Like other small GTPases, Ran exists in either a GTP-bound or GDP-bound state. RanGTP that resides in the nucleus and promotes nuclear export, while cytosolic RanGDP promotes import. The gradient of RanGTP across the nuclear membrane allows for appropriate movement of cargo proteins across the NPC as well as maintenance of the mitotic spindle (1-3). 
 Nuclear transport factor 2 (NTF2) regulates the subcellular distribution and function of Ran (4-5). The NTF2 homodimer facilitates the diffusion of RanGDP through NPCs via transient interactions with phenylalanine-glycine (FG) repeat domains on NPC proteins. NTF2 stabilizes the GDP-bound form of Ran until it is induced to dissociate by a nuclear factor in an ATP-dependent manor, thus allowing the guanine nucleotide exchange factor (GEF) RCC1 to mediate exchange of GDP for GTP on Ran (6-7).

1. Mattaj, I.W. and Englmeier, L. (1998) Annu Rev Biochem 67, 265-306.
2. Kalab, P. et al. (2002) Science 295, 2452-6.
3. Becskei, A. and Mattaj, I.W. (2003) Proc Natl Acad Sci USA 100, 1717-22.
4. Ribbeck, K. et al. (1998) EMBO J 17, 6587-98.
5. Steggerda, S.M. et al. (2000) Mol Biol Cell 11, 703-19.
6. Stewart, M. (2000) Cell Struct Funct 25, 217-25.
7. Yamada, M. et al. (2004) J Biol Chem 279, 36228-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.