Phospho-p95/NBS1 (Ser343) Antibody

• 产品编号：CST-3001S      品牌：CST       原厂货号：3001S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-p95/NBS1 (Ser343) Antibody能够检测内源性丝氨酸（343位）磷酸化的p95/NBS1 蛋白。该多克隆抗体是由合成的人源的针对p95/NBS1蛋白丝氨酸（343位）的磷酸化肽段免疫动物，利用蛋白A和多肽亲和层析技术生产的。 Nijmegen 断裂综合征(NBS)的主要特征有细胞周期检验点缺陷，生长迟缓，癌症倾向和离子辐射敏感性增强(1)。辐射修复DNA双链断裂依赖于多功能复合体，包括Rad50, Mre11和NBS1基因产物p95/NBS1 (也称作 p95 或nibrin) (2)。p95/NBS1是一种在细胞周期调控和DNA修复中发现的一种蛋白质，具有叉头相关结构域和重复序列的羧基末端(1,3)。共济失调毛细血管扩张症和NBS临床上以及细胞表型之间的重叠表明ATM和p95/NBS1在同一个生化通路中起作用。ATM和p95/NBS1相互作用并能够在暴露电离辐射之后磷酸化p95/NBS1蛋白丝氨酸343位(4-7)。

1. Chrzanowska, K.H. et al. (2012) Orphanet J Rare Dis 7, 13.
2. Lee, J.H. et al. (2013) J Biol Chem 288, 12840-51.
3. Hari, F.J. et al. (2010) EMBO Rep 11, 387-92.
4. Wang, H. et al. (2013) PLoS Genet 9, e1003277.
5. Zhao, S. et al. (2000) Nature 405, 473-7.
6. Wen, J. et al. (2013) Oncogene 32, 4448-56.

原厂资料：

Specificity / Sensitivity

Phospho-p95/NBS1 (Ser343) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser343 of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).

1. Chrzanowska, K.H. et al. (2012) Orphanet J Rare Dis 7, 13.
2. Lee, J.H. et al. (2013) J Biol Chem 288, 12840-51.
3. Hari, F.J. et al. (2010) EMBO Rep 11, 387-92.
4. Wang, H. et al. (2013) PLoS Genet 9, e1003277.
5. Zhao, S. et al. (2000) Nature 405, 473-7.
6. Wen, J. et al. (2013) Oncogene 32, 4448-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.