iNOS Antibody (Mouse Specific)

• 产品编号：CST-2982S      品牌：CST       原厂货号：2982S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

iNOS Antibody(Mouse Specific)能够检测内源性iNOS总蛋白水平。此多克隆抗体是通过合成鼠源对应的iNOS Ala1130位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。一氧化氮合酶(NOS)催化产生一氧化氮(NO)并在氧气和共因子作用下从L-精氨酸合成瓜氨酸。有三个家族成员已经得到鉴定: 神经元NOS (nNOS), 它主要在神经组织中发现; 可诱导NOS (iNOS), 它在肾和心脏血管系统中能够被干扰素gamma和脂多糖诱导;内皮NOS (eNOS),它主要在血管中表达(1)。NO在整个身体中作为一个信使分子而发挥多种作用，包括维持血管的完整性、动态平衡、染色体接合的可塑性、长期的势差现象、学习和记忆(2,3)。一氧化氮主要由iNOS产生，一氧化氮参与到了抵抗原生动物、细菌、真菌和病毒过程中。与组成型表达的eNOS 和 nNos不同，iNOS通常不在静止细胞中表达。iNOS受到细菌毒素LPS、巨噬细胞的炎症因子和其它细胞类型的诱导而转录。参与到iNOS转录的转录因子包括NF-κB, AP-1 和 STAT。不同的信号通路或者促进(Jak1/2, PKC, c-Raf, p38 MAP 激酶和 p44/42 MAP激酶)或者抑制(PI3 激酶) iNOS的表达依赖于刺激和细胞的种类(4)。

1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
2. Son, H. et al. (1996) Cell 87, 1015-10123.
3. Hawkins, R.D. (1996) Neuron 16, 465-467.
4. Bogdan, C. (2001) Nat Immunol 2, 907-16.

原厂资料：

Specificity / Sensitivity

iNOS Antibody (Mouse Specific) detects endogenous levels of total iNOS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide sourrounding Ala1130 of mouse iNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins such as LPS and proinflammatory cytokines in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).

1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
2. Son, H. et al. (1996) Cell 87, 1015-10123.
3. Hawkins, R.D. (1996) Neuron 16, 465-467.
4. Bogdan, C. (2001) Nat Immunol 2, 907-16.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.