Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody

• 产品编号：CST-2981S      品牌：CST       原厂货号：2981S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody识别包含Arg-X-Tyr/Phe-X-pSer基序的内源水平的蛋白质。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、其它基序中的磷酸化丝氨酸/苏氨酸残基发生交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该多克隆抗体用含Arg-X-Tyr/Phe-X-pSer肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。14-3-3蛋白是参与调解细胞生存、凋亡、增殖和检查点调控的一组高度保守的蛋白家族（1-5）。14-3-3的结合由包含磷酸化丝氨酸的蛋白质介导（6）。两种包含磷酸化丝氨酸的基序在简并磷酸化导向肽库技术中发现，分别为RSXS*XP和RXY/FXS*XP（6）。基序2（RXY/FXS*XP）在很多重要的调节蛋白包括cdc25A、cdc25B、PKCγ、IRS-1和BCR中发现（6）。尽管Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody可以结合14-3-3结合基序2，并且不需要+2位上的脯氨酸，它仍然是发现和鉴定潜在包含14-3-3结合基序2蛋白或其它含RXY/FXS*基序蛋白的有力工具。

1. Aitken, A. (1995) Trends Biochem Sci 20, 95-7.
2. Zha, J. et al. (1996) Cell 87, 619-28.
3. Piwnica-Worms, H. (1999) Nature 401, 535, 537.
4. Tzivion, G. et al. (1998) Nature 394, 88-92.
5. Xing, H. et al. (2000) EMBO J 19, 349-58.
6. Yaffe, M.B. et al. (1997) Cell 91, 961-71.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody detects endogenous levels of proteins containing the Arg-X-Tyr/Phe-X-pSer motif. This antibody does not cross-react with nonphosphorylated serine or phospho-threonine proteins with the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with Arg-X-Tyr/Phe-X-pSer-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (6). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR (6). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif.

1. Aitken, A. (1995) Trends Biochem Sci 20, 95-7.
2. Zha, J. et al. (1996) Cell 87, 619-28.
3. Piwnica-Worms, H. (1999) Nature 401, 535, 537.
4. Tzivion, G. et al. (1998) Nature 394, 88-92.
5. Xing, H. et al. (2000) EMBO J 19, 349-58.
6. Yaffe, M.B. et al. (1997) Cell 91, 961-71.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.