Erlin-1 Antibody

• 产品编号：CST-2958S      品牌：CST       原厂货号：2958S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Erlin-1 Antibody检测内源性人源erlin-1蛋白。通过人工合成人源erlin-1蛋白Gly320位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过多肽亲和层析纯化抗体。Erlin-1和erlin-2 (SPFH1 and SPFH2)是含有SPFH结构域的蛋白，它们属于抑制素家族(1,2)。erlin蛋白的N端区域含有ER靶向序列，该序列负责它们的蛋白定位到内质网（endoplasmic reticulum，ER）(3)。在内质网中，erlin-1 和erlin-2蛋白特别地与脂筏膜的脂筏微结构关联(3)。Erlin-1蛋白可能是涉及树枝状细胞激活(4) ，而erlin-2蛋白通过与内源性基底相互作用，已经显示调节内质网相关降解（ER-associated degradation，ERAD) 通路，导致它们的泛素化和降解(5)。

1. Browman, D.T. et al. (2007) Trends Cell Biol 17, 394-402.
2. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
3. Browman, D.T. et al. (2006) J Cell Sci 119, 3149-60.
4. Li, N. et al. (2000) Biochem Biophys Res Commun 279, 487-93.
5. Pearce, M.M. et al. (2007) J Biol Chem 282, 20104-15.

原厂资料：

Specificity / Sensitivity

Erlin-1 Antibody detects endogenous levels of total human erlin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly320 of human erlin-1. Antibodies are purified by peptide affinity chromatography.

Background

Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family (1,2). The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER) (3). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane (3). Erlin-1 may be involved in dentritic cell activation (4) and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation (5).

1. Browman, D.T. et al. (2007) Trends Cell Biol 17, 394-402.
2. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
3. Browman, D.T. et al. (2006) J Cell Sci 119, 3149-60.
4. Li, N. et al. (2000) Biochem Biophys Res Commun 279, 487-93.
5. Pearce, M.M. et al. (2007) J Biol Chem 282, 20104-15.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.