Cyclin D1 Antibody

• 产品编号：CST-2922S      品牌：CST       原厂货号：2922S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclin D1 Antibody能够识别内源性cyclin D1蛋白，且不与cyclin D家族其他成员发生交叉反应。该多克隆抗体是由合成的人源的针对cyclin D1蛋白羧基末端的肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。细胞周期蛋白依赖性蛋白激酶CDK4和CDK6的活性受三个因素的调控：T-环路磷酸化，周期蛋白伴侣(D-型周期蛋白)的丰度，与CDK抑制剂Cip/Kip 或 INK蛋白家族的关系(1)。失活的三元复合物cyclin D/CDK4 和p27 Kip1需要胞外有丝分裂刺激因子参与p27伴侣的释放和降解，伴随周期蛋白D的水平升高，通过限制点和pRb依赖性的S期进入影响细胞周期的进程(2)。活跃的cyclin D/CDK4复合体靶向视网膜母细胞瘤蛋白以利于磷酸化，促进可以激活G1/S期基因表达的转录因子E2F的释放(3)。生长因子退出后，通过下调蛋白表达和磷酸化依赖性蛋白降解，周期蛋白D的水平下降(4)。

1. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
2. Sherr, C.J. (1996) Science 274, 1672-1677.
3. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
4. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.

原厂资料：

Specificity / Sensitivity

Cyclin D1 Antibody detects endogenous levels of cyclin D1. It does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of cyclin D1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4).

1. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
2. Sherr, C.J. (1996) Science 274, 1672-1677.
3. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
4. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.