DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG? M2 Antibody) (Biotinylated)

• 产品编号：CST-2908S      品牌：CST       原厂货号：2908S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

DYKDDDDK标签抗体（生物素化） 可以检测细胞中内源性表达DYKDDDDK的蛋白。此抗体可以识别与目的蛋白氨基末端或羧基末端的融合的DYKDDDDK肽段（与Sigma公司的抗FLAG抗体识别相同的表位）。此种抗体结合的特异性不依赖于二价金属离子的存在。多克隆抗体是采用合成的DYKDDDDK肽段免疫动物而生产的(KLH-coupled) 。抗体采用蛋白A和亲和色谱法纯化。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。DYKDDDDK肽段作为常规的表位标签被广泛应用于表达载体中，此肽段可以通过与目的蛋白的氨基末端或羧基末端融合而被表达和检测到（1）。

Brizzard, B. L. et al. (1994) Biotechniques 16, 730-735.

原厂资料：

Specificity / Sensitivity

DYKDDDDK Tag Antibody (Biotinylated) detects transfected levels of DYKDDDDK tagged proteins. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

Brizzard, B. L. et al. (1994) Biotechniques 16, 730-735.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.