ERp57 (A484) Antibody

• 产品编号：CST-2887S      品牌：CST       原厂货号：2887S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ERp57 (A484) Antibody识别内源性的ERp57总蛋白。该多克隆抗体用与人类ERp57蛋白中Ala484位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。分泌蛋白合成后转位到内质网进行翻译后修饰和正确折叠。为了形成其天然构象，很多分泌蛋白需要形成分子间或分子内二硫键（1）。这个过程成为氧化蛋白折叠。二硫键异构酶（PDI）催化蛋白质天然活性形成的二硫键形成和异构变化（2）。其它驻内质网的拥有硫氧还蛋白同源性区域的蛋白，包括内质网应激蛋白57（ERp57），组成了PDI家族（2）。ERp57与calnexin和calreticulin（3）相互作用，可能在某些糖蛋白的二硫键异构中发挥作用（3）。

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Jessop, C.E. et al. (2007) EMBO J 26, 28-40.

原厂资料：

Specificity / Sensitivity

ERp57 (A484) Antibody detects endogenous levels of total ERp57 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala484 of human ERp57. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Jessop, C.E. et al. (2007) EMBO J 26, 28-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.