ERC1α (D1055) Antibody

• 产品编号：CST-2885S      品牌：CST       原厂货号：2885S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ERC1α (D1055) 抗体能够检测内源人的α 剪接形式ERC1蛋白。此多克隆抗体是通过合成人源对应的ERC1 C-末端周围的肽段来免疫动物而获得。此抗体特异性的针对α剪接形式。抗体是通过多肽亲和层析纯化。ERC1,是早先鉴定的ELKS(1), RAB6IP2 (2) 和 CAST (3)蛋白首字母缩略词，它是RIM结合蛋白，在神经递质的释放和其它类型细胞中的膜转运中发挥作用(2-5)。与GFP结合蛋白Rab6的相互作用表明它对高尔基体的膜转运有贡献(2)。除了与膜转运有关, ERC1被发现也是IκB 激酶(IKK)复合体的重要部分(IKK)，是NF-κB信号通路激活所必需的, 其中ERC1的作用可能是招募IκBα到IKK复合体上(6)。ERC1基因的可变剪切产生了2个具有不同C-末端的蛋白, 一个长的形式和一个短的形式，分别叫做ERC1α和ERC1β。ERC1α广泛的表达, 而ERC1β及其相关的家族成员ERC2只在脑中表达(4)。甲状腺乳头状癌已经鉴定是由于易位t(10;12)(p11;p13)使ERC1及其受体酪氨酸激酶Ret的融合导致了疾病的发生(1)。

1. Nakata, T. et al. (1999) Genes Chromosomes Cancer 25, 97-103.
2. Monier, S. et al. (2002) Traffic 3, 289-97.
3. Ohtsuka, T. et al. (2002) J Cell Biol 158, 577-90.
4. Wang, Y. et al. (2002) Proc Natl Acad Sci USA 99, 14464-9.
5. Ohara-Imaizumi, M. et al. (2005) Mol Biol Cell 16, 3289-300.
6. Ducut Sigala, J.L. et al. (2004) Science 304, 1963-7.

原厂资料：

Specificity / Sensitivity

ERC1α (D1055) Antibody detects endogenous levels of the α isoform of human ERC1.Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human ERC1 protein, specific for the α isoform. Antibodies were purified by peptide affinity chromatography.Background

ERC1, an acronym named for previous protein names ELKS (1), RAB6IP2 (2) and CAST (3), is a RIM-binding protein that plays a role in neurotransmitter release and general membrane trafficking in other cell types (2-5). Interaction with the GTP-binding protein Rab6 suggests that it contributes to membrane traffic at the Golgi (2). In addition to its association with membrane trafficking, ERC1 has also been found as an essential part of the IκB kinase (IKK) complex required for the activation of NF-κB, perhaps by recruiting IκBα to the IKK complex (6). Alternative splicing of ERC1 generates 2 proteins with a divergent carboxy terminus, a long and a short form termed ERC1α and ERC1β, respectively. ERC1α is widely expressed, whereas ERC1β and a related family member ERC2 are expressed in the brain (4). Papillary thyroid carcinomas have been identified with the translocation t(10;12)(p11;p13) resulting in a fusion between ERC1 and the receptor tyrosine kinase Ret (1).

1. Nakata, T. et al. (1999) Genes Chromosomes Cancer 25, 97-103.
2. Monier, S. et al. (2002) Traffic 3, 289-97.
3. Ohtsuka, T. et al. (2002) J Cell Biol 158, 577-90.
4. Wang, Y. et al. (2002) Proc Natl Acad Sci USA 99, 14464-9.
5. Ohara-Imaizumi, M. et al. (2005) Mol Biol Cell 16, 3289-300.
6. Ducut Sigala, J.L. et al. (2004) Science 304, 1963-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.