LITAF Antibody

• 产品编号：CST-2879S      品牌：CST       原厂货号：2879S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LITAF抗体能够检测内源人LITAF蛋白总体水平。此多克隆抗体是通过合成人源对应的LITAF N-末端周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析法纯化。LITAF (PIG7/SIMPLE)蛋白参与到调控炎症因子TNF-α的调控中(1-3)。LITAF是作为转录因子被鉴定，它通过与TNF-α启动子的相互作用而参与到LPS诱导的TNF-α表达过程中(1,4)。同样的蛋白,称作PIG7,在p53调控模式中被独立的描述(2)。是LITA潜在的可变剪切的另一种形式叫做SIMPLE, 编码一个具有独特C-末端的蛋白(3)。研究LITAF缺陷型小鼠表明LITAF在对LPS的响应中和在调控炎症因子过程中发挥了重要的作用(5)。LITAF的调控是通过TLR通路而被p38α磷酸化并导致其入核(5)。LITAF/SIMPLE基因的突变与常染色体显性遗传的脱髓鞘形式的腓骨肌萎缩症有关(6)。

1. Myokai, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 4518-4523.
2. Zhu, J. et al. (1999) Oncogene 18, 2149-2155.
3. Moriwaki, Y. et al. (2001) J. Biol. Chem. 276, 23065-23076.
4. Tang, X. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4096-4101.
5. Tang, X. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 13777-13782.
6. Street, V.A. et al. (2003) Neurology 60, 22-26.

原厂资料：

Specificity / Sensitivity

LITAF Antibody dectects endogenous levels of total human LITAF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LITAF protein. Antibodies were purified by peptide affinity chromatography.

Background

LITAF (PIG7/SIMPLE) is protein that contributes to the regulation of the inflammatory cytokine TNF-α (1-3). LITAF was identified as a transcription factor involved in LPS-induced TNF-α expression through interaction with the TNF-α promoter (1,4). The same protein, called PIG7, was independently described in a model for p53 regulation (2). A putative alternative spiced form of LITAF, named SIMPLE, encodes a protein with a unique carboxyl terminus (3). Studies on LITAF-deficient mice demonstrate that LITAF plays a significant role in the regulation of several inflammatory cytokines in response to LPS (5). The regulation of LITAF can occur through phosphorylation by p38α via the TLR pathway that leads to its nuclear translocation (5). Mutation in the LITAF/SIMPLE gene has been associated with an autosomal dominant demyelinating form of Charcot-Marie-Tooth disease (6).

1. Myokai, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 4518-4523.
2. Zhu, J. et al. (1999) Oncogene 18, 2149-2155.
3. Moriwaki, Y. et al. (2001) J. Biol. Chem. 276, 23065-23076.
4. Tang, X. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4096-4101.
5. Tang, X. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 13777-13782.
6. Street, V.A. et al. (2003) Neurology 60, 22-26.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.