CASK Antibody

• 产品编号：CST-2878S      品牌：CST       原厂货号：2878S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CASK Antibody 兔多抗识别内源性的总CASK蛋白。该多克隆抗体由合成的人类CASK蛋白氨基末端序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。CASK是一个具有钙/钙调素依赖性蛋白激酶结构域，一个SH3结构域，一个鸟苷酸激酶同源结构域（GUK），和一个PDZ结构域的接头蛋白。CASK将细胞骨架和信号分子链接在跨膜蛋白上。特别地，CASK与neurexin结合，稳定突触前和突触后结构（1）。大多数CASK蛋白（?80％??）存在于是细胞质中，一部分蛋白质进入细胞核，作为转录的共激活因子（2）。CASK插入性突变的转基因小鼠在出生24小时内死亡（3）。

1. Hata, Y. et al. (1996) J Neurosci 16, 2488-94.
2. Hsueh, Y.P. et al. (2000) Nature 404, 298-302.
3. Laverty, H.G. and Wilson, J.B. (1998) Genomics 53, 29-41.

原厂资料：

Specificity / Sensitivity

CASK Antibody detects endogenous levels of total CASK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminus of CASK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CASK is an adaptor protein with a calcium/calmodulin-dependent protein kinase domain, an SH3 domain, a guanylate kinase homology domain (GUK), and a PDZ domain. CASK links transmembrane proteins to the cytoskeleton and signaling molecules. In particular, CASK binds to neurexin to stabilize pre- and post-synaptic structures (1). While most CASK protein (~80%) is cytoplasmic, a portion of the protein enters the nucleus, where it acts as a transcriptional coactivator (2). Transgenic mice with CASK insertional mutations die within 24 hours of birth (3).

1. Hata, Y. et al. (1996) J Neurosci 16, 2488-94.
2. Hsueh, Y.P. et al. (2000) Nature 404, 298-302.
3. Laverty, H.G. and Wilson, J.B. (1998) Genomics 53, 29-41.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.