Survivin (71G4B7) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-2866S      品牌：CST       原厂货号：2866S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Survivin (71G4) Rabbit mAb (Alexa Fluor^® 647 Conjugate)能够检测内源性水平survivin总蛋白。该单克隆抗体是采用合成的人源survivin蛋白Cys60周围残基相对应的肽段免疫动物而制备的。在优化条件下，该抗体连接了Alexa Fluor^® 647接头，F/P比为2-6。Alexa Fluor^® 647染料被红光（例如633 nm的He-Ne激光）最大限度激发。Alexa Fluor^® 647染料标记抗体产生明亮的远红荧光，在665 nm处为峰值。该CST抗体标记了Alexa Fluor^® 647荧光染料，通过了内部测试可以直接用于人细胞的流式细胞试验。不带标记的#2808产品与人、小鼠和大鼠有反应。CST期待Survivin (71G4E) Rabbit mAb 兔单抗 (Alexa Fluor^® 647 Conjugate)也能识别这些物种。Survivin是一个在胎儿发育和肿瘤细胞恶变过程中高表达的抗凋亡蛋白，大小为16 kDa（1）。Survivin结合并抑制caspase-3，通过抑制细胞凋亡调控和促进细胞分裂，从而来控制细胞周期的G2/M-期临界点（2,3）。这个调控过程需要Survivin在P34 CDC2激酶的作用下，在Thr34位发生磷酸化（4）。通过基因打靶实验和Survivin反义RNA实验，应用Thr34磷酸化缺陷的survivin突变体，已经证明了survivin能够抑制肿瘤的生长（5,6）。

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

原厂资料：

Specificity / Sensitivity

Survivin (71G4E) Rabbit mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total survivin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 60 of human survivin. The antibody was conjugated to Alexa Fluor^® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor^® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor^® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody, #2808 reacts with survivin from human, mouse, and rat. CST expects that Survivin (71G4E) Rabbit mAb (Alexa Fluor^® 647 Conjugate) will also recognize survivin in these species.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze.