4E-BP2 Antibody

• 产品编号：CST-2845S      品牌：CST       原厂货号：2845S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

4E-BP2 Antibody检测内源性非磷酸化4E-BP2总蛋白水平。这个抗体不能和4E-BP1蛋白发生有意义的交叉反应。通过人工合成人源4E-BP2蛋白羧基末端序列相应多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化多克隆抗体。翻译抑制蛋白4E-BP1 (又称 PHAS-1)通过结合翻译起始因子eIF4E蛋白从而抑制帽子结构依赖的翻译。4E-BP1的过磷酸化干扰上述结合并且导致激活帽子结构依赖的翻译（1）。PI3 kinase/Akt通路和FRAP/mTOR激酶通路都调节4E-BP1蛋白的激活（2,3）。在体内4E-BP1多个位点残基被磷酸化（4）。通过FRAP/mTOR蛋白使4E-BP1在Thr37和Thr46位点磷酸化不能阻止它eIF4E结合，但这被认为是为随后在4E-BP1的Ser65和Thr70位点磷酸化进行准备（5）。4E-BP2和4E-BP3与4E-BP1蛋白有高度同源性，其中保守序列包括主要的FRAP/mTOR依赖的磷酸化位点。虽然4E-BP2蛋白磷酸化还没有被深入的研究，但是初步的数据认为有类似于调节4E-BP1的途径去调节4E-BP2的磷酸化（6）。

1. Pause, A. et al. (1994) Nature 371, 762-7.
2. Brunn, G.J. et al. (1997) Science 277, 99-101.
3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.
6. Lin, T.A. and Lawrence, Jr, J.C. (1996) J. Biol. Chem. 271, 30199-30204.

原厂资料：

Specificity / Sensitivity

4E-BP2 Antibody detects endogenous levels of total 4E-BP2, independent of phosphorylation. This antibody does not cross-react significantly with 4E-BP1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy-terminus of human 4E-BP2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).4E-BP2 and 4E-BP3 share high sequence homology with 4E-BP1, including conservation of the major FRAP/mTOR-dependent phosphorylation sites. Preliminary data suggests that phosphorylation of 4E-BP2 is regulated in a similar manner to that of 4E-BP1, although phosphorylation of this protein has not been as extensively studied (6).

1. Pause, A. et al. (1994) Nature 371, 762-7.
2. Brunn, G.J. et al. (1997) Science 277, 99-101.
3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.
6. Lin, T.A. and Lawrence, Jr, J.C. (1996) J. Biol. Chem. 271, 30199-30204.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody