SHIP2 (C76A7) Rabbit mAb

• 产品编号：CST-2839S      品牌：CST       原厂货号：2839S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SHIP2 (C76A7) Rabbit mAb 能够检测内源SHIP2蛋白总体水平。单克隆抗体是通过合成人源对应的SHIP2 Ala1083位点周围的肽段来免疫动物而获得。包含SH2的肌糖磷酸酶1(SHIP1)是造血组织中的磷酸酶，水解磷脂酰肌醇-3,4,5-三磷酸盐成磷脂酰肌醇-3,4-二磷酸盐(1)。SHIP1是细胞质磷酸酶在其N-末端有SH2区域 和C-末端的两个NPXY Shc结合区域(1,2)。受体交叉结合后, SHIP首先被招募到膜结合处，通过其SH2区域结合到ITIM域的磷酸化酪氨酸上(2),这在酪氨酸磷酸化NPXY域之后(2)。细胞膜的重新定位和NPXY域的磷酸化对SHIP1的调控功能非常重要(3-5)。它通过PI3K和Akt通路影响钙流，细胞的存活，生长，细胞周期和凋亡(3-5)。Tyr1021定位于SHIP1的一个NPXY域内, 而且其磷酸化对SHIP1功能是非常重要的(6)。SHIP2是SHIP1的同源基因,在心脏，骨骼肌，胎盘中高表达(7)。SHIP2负调控胰岛素信号(8) ,SHIP2的多态性与高血糖症有关(9)。最近的研究也表明SHIP2作为肥胖症和2型糖尿病的治疗靶标(10,11)。

1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
7. Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
8. Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
9. Ishida, S. et al. (2006) Pancreas 33, 63-7.
10. Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
11. Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809.

原厂资料：

Specificity / Sensitivity

SHIP2 (C76A7) Rabbit mAb detects endogenous levels of total SHIP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1083 of human SHIP2.

Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta (7). SHIP2 negatively regulates insulin signaling (8) and polymorphisms in SHIP2 have been linked to hyperglycemia (9). Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes (10,11).

1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
7. Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
8. Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
9. Ishida, S. et al. (2006) Pancreas 33, 63-7.
10. Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
11. Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.