Phospho-MARCKS (Ser152/156) Antibody

• 产品编号：CST-2741S      品牌：CST       原厂货号：2741S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MARCKS(Ser152/156)抗体识别内源性的Ser152和Ser156磷酸化的MARCKS蛋白。该多克隆抗体用与大鼠MARCKS中Ser152/156位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。该多克隆抗体用与大鼠MARCKS中Ser152/156位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

原厂资料：

Specificity / Sensitivity

Phospho-MARCKS (Ser152/156) Antibody detects endogenous levels of MARCKS only when phosphorylated at serines 152 and 156.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser152/156 of rat MARCKS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody