Fes Antibody

• 产品编号：CST-2736S      品牌：CST       原厂货号：2736S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fes Antibody检测内源性Fes总蛋白。该抗体不能与其它蛋白质发生交叉反应。通过人工合成人源Fes蛋白Thr421位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Fes/Fps和Fer蛋白是唯一的细胞质蛋白酪氨酸激酶家族的仅有的两个成员(1,2)。Fps和Fer 家族含有一个中心Src homology-2 (SH2)结构域和一个羧基末端酪氨酸激酶催化结构域。通过氨基末端Fer/CIP4同源性和卷曲螺旋结构的存在，这些蛋白与来自细胞质蛋白酪氨酸激酶亚家族的其它成员有结构性区别 (3)。Fes/Fps起初被鉴定作为一个来自鸟类的 (Fps)和猫科的(Fes)逆转录酶病毒的致癌基因。人源c-Fes蛋白已经涉及骨髓的、血管内皮和神经元细胞的分化。突变可能激活Fps激酶，从而导致癌症(4)。 然而，最近数据充分证明 c-Fes蛋白酪氨酸激酶是一个肿瘤抑制因子，而不是在结肠直肠癌中的主导的致癌基因 (5)。

原厂资料：

Specificity / Sensitivity

Fes Antibody detects endogenous levels of Fes proteins. This antibody does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr421 of human Fes. Antibodies are purified by protein A and peptide affinity chromatography.  

Background

Fes/Fps and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases (1,2). Fps and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains (3). Fes/Fps was originally identified as an oncogene from avian (Fps) and feline (Fes) retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. Mutations may activate the Fps kinase and thereby contribute to cancer (4). However, recent data strongly suggests that the c-Fes protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer (5).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody