Numb Antibody

• 产品编号：CST-2733S      品牌：Cell Signaling Technology       原厂货号：2733S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Numb抗体识别内源的总Numb 蛋白.多克隆抗体是通过合成的肽段免疫动物产生。这种合成的肽段与邻近人源Numb蛋白的Phe482氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化.Numb包含一个氨基末端磷酸化酪氨酸结合（PTB）结构域和C末端对α-衔接蛋白和EH（Eps15 同源物）结构域包含蛋白的内吞结合基序，显示在细胞吞噬中的作用（1,2）有四个哺乳动物Numb剪接异构体，差异表达，并有各自独特功能（3-5）。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用（6），Numb蛋白在细胞分裂时不对称的进入了某个子代细胞，并对产生的两个子细胞中的Notch信号通路和细胞命运都有不同反应（7,8）。Numb蛋白的定位可能被G蛋白偶联受体和PKC信号调控（9）。

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

原厂资料：

Specificity / Sensitivity

Numb Antibody detects endogenous levels of total Numb protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody