Phospho-Lyn (Tyr507) Antibody

• 产品编号：CST-2731S      品牌：CST       原厂货号：2731S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Lyn (Tyr507)抗体只能够检测内源水平在Tyr507位点磷酸化的Lyn蛋白。此抗体可能与phospho-Lck (Tyr505)和phospho-Src (Tyr527)交叉反应，因为高度的序列相似。此多克隆抗体是通过合成人源对应的Lyn Tyr507位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。Lyn作为 Src 家族的一员在造血细胞中高表达(1)。据报道两个酪氨酸残基对Src家族中的酪氨酸激酶调控发挥了及其重要的作用。催化区域 Tyr396位点的自磷酸化 (等同于 Src 的 Tyr416 位点 )与酶活性有很大的关联。 Csk-介导的C-末端Tyr507位点的磷酸化 (等同于 Src 的 Tyr527 位点)能使酶失活。 酪氨酸激酶的磷酸化和 Lyn的激活与细胞表面受体BCR和 CD40 有关(2-4)。用基因敲除老鼠研究表明Lyn缺失的影响是刺激B细胞而对BCR的刺激过敏反应(5-7), 这就表明Lyn在体内的作用主要是在B细胞应答的下游。Lyn也参与到控制特殊B细胞类群的迁移和发育(8)。

1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-Lyn (Tyr507) Antibody detects endogenous levels of Lyn only when phosphorylated at Tyr507. The antibody may cross-react with phospho-Lck (Tyr505) and phospho-Src (Tyr527) due to high sequence homology.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr507 of human Lyn. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.