Zap-70 (136F12) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-2707S      品牌：CST       原厂货号：2707S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)抗体能够检测内源Zap-70蛋白总体水平。此单克隆抗体是通过合成人源对应的Zap-70 N-末端周围的肽段来免疫动物而获得。此抗体在优化的条件下与Alexa Fluor® 647 偶联，F/P 比例为2-6。Alexa Fluor® 647荧光染料的最大激发光是红光(e.g. 633 nm He-Ne laser)。与此染料偶联的抗体产生的远红外荧光在665 nm有最大的吸收峰。CST公司生产的此抗体与Alexa Fluor® 647荧光染料偶联，并通过人源细胞的流式细胞仪的测试。Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

原厂资料：

Specificity / Sensitivity

Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total Zap-70 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.