CK2α Antibody

• 产品编号：CST-2656S      品牌：CST       原厂货号：2656S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源性CK2α 1总蛋白，最有可能与CK2α发生交叉反应。该多克隆抗体是由合成的人源的针对CK2α1蛋白羧基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。CK2(以前称作酪蛋白激酶 II)是一种高度保守的具有300多个作用底物的蛋白激酶，能够调节细胞生长、细胞死亡和细胞存活。CK2在响应紫外照射诱导的DNA损伤中被发现，它能够靶向XRCC1 (1) 和BRCA1 (2)并能调节p53肿瘤抑制基因的功能(3)。此外，CK2在NF-κB的激活过程中发挥着关键的作用(4)。紫外照射能够刺激CK2介导的IκBα中几个羧基末端残基的磷酸化，引起IκBα的蛋白酶体降解和释放以及活化的NF-κB的核转移。CK2在许多肿瘤和神经退行性疾病如阿尔茨海默氏症和帕金森症中功能紊乱(6)。结构上讲，CK2是一个多聚化的蛋白复合体，由两个催化亚基(α or α')和两个调节亚基β组成(7)。CK2分布广泛，明显的组成活跃(7)。尽管CK2α 和CK2β上已鉴定出细胞周期依赖性的Ser-Pro磷酸化位点，Src-相关激酶 c-Fgr作用的255位酪氨酸磷酸化似乎对CK2α的活性有最大的影响(8,9)。

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

原厂资料：

Specificity / Sensitivity

This antibody detects endogenous levels of total CK2α 1 protein. This antibody may cross-react with CK2α prime.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues near the carboxy-terminus of human CK2α1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimer's and Parkinson's diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α') and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9). 

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.