Pan-Calcineurin A Antibody

• 产品编号：CST-2614S      品牌：CST       原厂货号：2614S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体识别内源性的全部Calcineurin A（α亚型）蛋白。此抗体也可识别Calcineurin A的β和γ亚型。此抗体不予蛋白磷酸酶1或2A发生交叉反应。该多克隆抗体用与人类Calcineurin A（α亚型）羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。钙调神经磷酸酶，又称蛋白磷酸酶2B（PP2B），是一种钙调蛋白依赖的、钙激活的丝氨酸/苏氨酸蛋白磷酸酶，由一个催化亚基（calcineurin A）和一个紧密结合的调节亚基（calcineurin B）组成（1）。钙调神经磷酸酶与蛋白磷酸酶1和2A高度同源。钙调神经磷酸酶B，和钙调蛋白一样，包含有4个EF手性钙结合模体。钙调神经磷酸酶信号与很多细胞过程有关，包括细胞周期调控、应激反应和细胞凋亡，是心血管和骨骼肌肉正常发育所必需（2,3）。钙调神经磷酸酶介导活化T细胞核因子（NFAT）转录因子的磷酸化，是NFAT激活核易位以及T淋巴细胞早期基因表达所必需（2,3）。钙调神经磷酸酶是免疫抑制剂环孢菌素A和FK506的靶点，两者都能在T细胞受体参与后阻止静息T细胞的活化（2,3）。环孢菌素A和FK506可分别结合immunophilins，cyclophilin和FKBP，immunophilin-药物复合体可结合钙调神经磷酸酶，阻断其与底物的结合。

1. Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.
2. Kahl, C.R. and Means, A.R. (2003) Endocr. Rev. 24, 719-36.
3. Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Pig

Specificity / Sensitivity

This antibody detects endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. The antibody does not cross-react with protein phosphatase 1 or 2A.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs. 
 Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.

1. Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.
2. Kahl, C.R. and Means, A.R. (2003) Endocr. Rev. 24, 719-36.
3. Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.