PP1α Antibody

• 产品编号：CST-2582S      品牌：CST       原厂货号：2582S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PP1α Antibody能够检测内源性PP1α总蛋白。该抗体不与PP1δ或PP1γ发生交叉反应。该多克隆抗体是由合成的人源的针对 PP1alpha蛋白氨基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。1型蛋白磷酸酶(PP1)是真核细胞中一种高度保守的丝氨酸/苏氨酸磷酸酶。PP1具有四个异构体：PP1α, PP1δ, PP1γ1 和PP1γ2 (1)。PP1的一个生物学功能是参与细胞周期调节(1)。有描述认为PP1在有丝分裂期能够去磷酸化Rb 和cdc25(2,3)。Cdc20介导的PP1α第320位苏氨酸细胞周期依赖性磷酸化能够抑制PP1α的活性(4)。

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

原厂资料：

Specificity / Sensitivity

PP1α Antibody detects endogenous levels of total PP1α protein. This antibody does not cross-react with PP1δ or PP1γ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP1alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4). 

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.