Phospho-PP1α (Thr320) Antibody

• 产品编号：CST-2581S      品牌：CST       原厂货号：2581S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PP1α (Thr320) Antibody能够检测内源性苏氨酸（320位）磷酸化的PP1α蛋白。该抗体也许与其他位点磷酸化的PP1蛋白的磷酸化δ或γ亚基发生交叉反应。该多克隆抗体是由合成的人源的针对PP1α蛋白苏氨酸（320位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。 1型蛋白磷酸酶(PP1)是真核细胞中一种高度保守的丝氨酸/苏氨酸磷酸酶。PP1具有四个异构体：PP1α, PP1δ, PP1γ1 和PP1γ2 (1)。PP1的一个生物学功能是参与细胞周期调节(1)。有描述认为PP1在有丝分裂期能够去磷酸化Rb 和cdc25(2,3)。Cdc20介导的PP1α第320位苏氨酸细胞周期依赖性磷酸化能够抑制PP1α的活性(4)。

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

原厂资料：

Specificity / Sensitivity

Phospho-PP1α (Thr320) Antibody detects PP1α only when phosphorylated at Thr320. This antibody may cross-react with the phosphorylated δ or γ isoforms of PP1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4). 

1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody