GLI1 Antibody

• 产品编号：CST-2553S      品牌：CST       原厂货号：2553S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GLI1抗体识别内源性的总GLI1蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与人源GLI1蛋白邻近Gly420氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化.GLI1 首先在恶性神经胶质瘤中被确定为一种扩增的基因（1）它能与逆转录病毒协作转化原代细胞（2）。GlI属于Kruppel家族锌指蛋白，包括三种哺乳类GLI蛋白：GLI1, GLI2 和GLI3（3）。这些GLI蛋白与果蝇同源体Ci(Cubitus interruptus)相似，是由Hedgehog信号通路激活的转录因子。Hedgehog信号通路对动物发育起重要作用，并在多种癌症中异常激活（4,5）.GLI1自身是Hedgehog信号通路转录靶标（6-8）在癌症研究中被用作Hedgehog信号通路标志物.

1. Kinzler, K.W. et al. (1987) Science 236, 70-3.
2. Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
3. Kinzler, K.W. et al. (1988) Nature 332, 371-4.
4. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
5. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
6. Lee, J. et al. (1997) Development 124, 2537-2552.
7. Dahmane, N. et al. (1997) Nature 389, 876-881.
8. Dai, P. et al. (1999) J. Biol. Chem. 274, 8143-8152.
9. Watkins, D.N. et al. (2003) Nature 422, 313-317.
10. Karhadkar, S.S. et al. (2004) Nature 431, 707-712.

原厂资料：

Specificity / Sensitivity

GLI1 Antibody detects endogenous levels of total GLI1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human GLI1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2 and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Ci (Cubitus interruptus) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).

1. Kinzler, K.W. et al. (1987) Science 236, 70-3.
2. Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
3. Kinzler, K.W. et al. (1988) Nature 332, 371-4.
4. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
5. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
6. Lee, J. et al. (1997) Development 124, 2537-2552.
7. Dahmane, N. et al. (1997) Nature 389, 876-881.
8. Dai, P. et al. (1999) J. Biol. Chem. 274, 8143-8152.
9. Watkins, D.N. et al. (2003) Nature 422, 313-317.
10. Karhadkar, S.S. et al. (2004) Nature 431, 707-712.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody