AP-2β Antibody

• 产品编号：CST-2509S      品牌：CST       原厂货号：2509S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AP-2β Antibody能够检测内源 AP-2β 总蛋白。该多克隆抗体是采用合成的人源AP-2β 序列对应的多肽来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。序列特异的转录因子激活蛋白2β (AP-2β)是肾脏正常发育所必需的(1)。AP-2β敲除小鼠由于肾脏多囊疾病在出生后1-2天就死亡(1)。在这些基因敲除的小鼠中，在胚胎发育的最后阶段，肾脏发育期间存在大量的细胞凋亡现象(1)。过表达AP-2β基因能够抑制 c-myc 诱导的凋亡, 这表明了这个转录因子在细胞存活中的作用(1)。另外，过表达AP-2β也表明了在脂肪细胞中不正常的胰岛素信号，因此此基因可能也是与肥胖症相关的潜在的基因之一(2)。

1. Moser, M. et al. (1997) Genes Dev. 11, 1938-1148.
2. Tao, Y. et al. (2006) Endocrinology 147, 1685-1696.

原厂资料：

Specificity / Sensitivity

AP-2β Antibody detects endogenous levels of total AP-2β protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human AP-2β. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The sequence-specific transcription factor activator protein 2β (AP-2β) is required for normal kidney development (1). AP-2β knockout mice die 1-2 days after birth due to polycystic kidney disease (1). Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1). Overexpressed AP-2β has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1). In addition, overexpression of AP-2β is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2).

1. Moser, M. et al. (1997) Genes Dev. 11, 1938-1148.
2. Tao, Y. et al. (2006) Endocrinology 147, 1685-1696.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.