PTCH2 (G1191) Antibody

• 产品编号：CST-2470S      品牌：CST       原厂货号：2470S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PTCH2 (G1191)抗体识别转染水平的PTCH2蛋白的水平，它不识别转染水平的PTCH1蛋白 。多克隆抗体是通过合成的肽段免疫动物产生的。这种合成的肽段与邻近人源PTCH2蛋白的Gly1191（预测是胞内）氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化 。

Patched1 和 2 (PTCH1 和 PTCH2)是十二次跨膜蛋白，起着对Hedgehog家族蛋白成员接收的受体作用（1-4）。Hedgehog蛋白不在时，PTCH抑制活性信号受体Smoothened (Smo)，这样Hedgehog信号通路处于关闭状态（5,6）。去激活突变，修复PTCH1抑制Smo的能力，被频繁发现在痣样基底细胞癌综合征病人身上（7,8）。PTCH蛋白有固醇传感结构域（SSD）,也在一些胆固醇稳态2中起作用的蛋白中发现，如HMGCR（3羟基3甲戊二酰辅酶A还原酶）和SCAP （甾醇调节成分绑定蛋白剪切激活蛋白）。但是，SSD在Patched蛋白作用还是未知（9,10）。

1. Stone, D.M. et al. (1996) Nature 384, 129-134.
2. Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
3. Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
5. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
7. Hahn, H. et al. (1996) Cell 85, 841-851.
8. Johnson, R.L. et al. (1996) Science 272, 1668-1671.
9. Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.

原厂资料：

Specificity / Sensitivity

PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region (predicted to be intracellular) surrounding residue Gly1191 of human PTCH2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).

1. Stone, D.M. et al. (1996) Nature 384, 129-134.
2. Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
3. Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
5. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
7. Hahn, H. et al. (1996) Cell 85, 841-851.
8. Johnson, R.L. et al. (1996) Science 272, 1668-1671.
9. Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.