APP/β-Amyloid (NAB228) Mouse mAb

• 产品编号：CST-2450P      品牌：Cell Signaling Technology       原厂货号：2450P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APP/beta-Amyloid (NAB228) Mouse mAb 鼠单抗识别内源性的APP/β-淀粉样蛋白。虽然这种抗体能够识别磷酸化和非磷酸化形式的目标蛋白质，但已证明其更倾向于在某些系统中的磷酸化形式。该单克隆抗体用β-淀粉样蛋白和β-淀粉样蛋白的抗原决定簇氨基酸末端免疫动物产生。

淀粉样蛋白β（A4）前体蛋白（APP）是100-140 kDa的跨膜糖蛋白，存在几个亚型（1）。APP的氨基酸序列中包含的淀粉样蛋白域（A-β），它可以通过两步的蛋白裂解反应释放出来（1）。释出的A-β片段在细胞外沉积积累，形成在阿尔茨海默病中淀粉样斑块的主要组成部分（1）。APP可以在多个位点被磷酸化，进而影响其蛋白裂解和分泌过程。（2-5）细胞周期蛋白依赖性激酶介导的Thr688（对应于APP695亚型）磷酸化是细胞周期依赖性的，在G2/M期达到峰值（4）。APP的Thr668磷酸化形式存在于成年鼠大脑和相应的培养神经细胞及其分化细胞中（5,6）。

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig

Specificity / Sensitivity

APP/beta-Amyloid (NAB228) Mouse mAb detects endogenous levels of APP/beta-Amyloid protein. Although this antibody recognizes both the phospho and non-phospho forms of the protein, it has been shown to prefer the phosphorylated form in some systems.

Source / Purification

Monoclonal antibody is produced by immunizing animals with beta-amyloid and the epitope maps to the amino terminus of beta-amyloid (Lee et al., 2003).

Background

Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody