eIF4H Antibody

• 产品编号：CST-2444S      品牌：CST       原厂货号：2444S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eIF4H Antibody检测内源性eIF4H总蛋白（无论长的还是短的异构体)水平。通过人工合成人源eIF4H蛋白相应多肽片段去免疫动物从而制备出多克隆抗体。抗体通过蛋白A和多肽亲和层析纯化。

众多的细胞因子参于了蛋白质翻译的起始。真核生物翻译起始因子4H (eIF4H)从兔网织红细胞裂解物中提取并纯化，并且显示在缺失eIF4F和eIF4B测试中具有激活翻译的作用(1)。eIF4H通过翻译起始因子eIF4A和eIF4B催化诱导RNA依赖的ATP水解作用(1,2)。eIF4H还能刺激翻译起始速率和eIF4A介导mRNA二级结构的打开(3)。eIF4H和herpes simplex virus shutoff protein (Vhs)相互作用对于Vhs介导的mRNA降解十分重要(4)。第7号染色体大面积缺失包括eIF4H基因导致Williams-Beuren Syndrome (WBS)发生，这疾病是一个常染色体显性紊乱可诱发心血管疾病，精神紊乱和特异的面部表情(5)。

1. Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
2. Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
3. Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
4. Sarma, N. et al. (2008) J Virol 82, 6600-9.
5. Osborne, L.R. et al. (1996) Genomics 36, 328-36.

原厂资料：

Specificity / Sensitivity

eIF4H Antibody detects endogenous levels of total eIF4H protein (both long and short isoforms).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human eIF4H. Antibodies are purified by protein A and peptide affinity chromatography.

Background

A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5).

1. Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
2. Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
3. Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
4. Sarma, N. et al. (2008) J Virol 82, 6600-9.
5. Osborne, L.R. et al. (1996) Genomics 36, 328-36.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.