Notch4 (L5C5) Mouse mAb

• 产品编号：CST-2423S      品牌：CST       原厂货号：2423S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Notch4 (L5C5)Mouse mAb小鼠单抗识别转染水平的人Notch4蛋白的胞内区域.单抗由重组蛋白免疫动物产生，该重组蛋白包含人源Notch4蛋白的锚蛋白复制结构域。Notch1是一个在细胞发育和命运决定过程中发挥功能的跨膜蛋白（1）。在notch1成熟过程中，notch1分子细胞外结构域会被一个成对碱性氨基酸蛋白酶样的转化酶剪切（2）。在与配体如Delta1结合或是细胞外钙离子耗竭后，notch1的羧基端被释放并随后在Gly1743 和 Val1744之间被切开（3,4）。结果导致细胞内处于活化状态的notch1片段转运至细胞核内并转录激活。Notch4 主要在内皮细胞表达，加工过程与Notch1相似（5-7）。小鼠Notch4基因（也称Int-3）经常作为诱导乳腺癌的小鼠乳腺癌病毒（MMTV）的靶标（6,8）。Notch4的胞内结构域与Smad3结合并抑制TGF-β 通路（9）.

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. Uyttendaele, H. et al. (1996) Development 122, 2251-2259.
6. Shirayoshi, Y. et al. (1997) Genes Cells 2, 213-224.
7. Baron, M. (2003) Semin. Cell Dev. Biol. 14, 113-119.
8. Gallahan, D. and Callahan, R. (1997) Oncogene 14, 1883-1890.
9. Sun, Y. et al. (2005) Oncogene 24, 5365-5374.

原厂资料：

Specificity / Sensitivity

Notch4 (L5C5) Mouse mAb detects levels of the transfected intracellular region of human Notch4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the Ankyrin repeat domain of human Notch4.

Background

Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1 (5-7). The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors (6,8). The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling (9).

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. Uyttendaele, H. et al. (1996) Development 122, 2251-2259.
6. Shirayoshi, Y. et al. (1997) Genes Cells 2, 213-224.
7. Baron, M. (2003) Semin. Cell Dev. Biol. 14, 113-119.
8. Gallahan, D. and Callahan, R. (1997) Oncogene 14, 1883-1890.
9. Sun, Y. et al. (2005) Oncogene 24, 5365-5374.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.