Rab11b Antibody

• 产品编号：CST-2414S      品牌：CST       原厂货号：2414S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab11b Antibody识别内源性的Rab11b总蛋白。该多克隆抗体用与人类Rab11b中Arg184位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Rab11a、Rab11b和Rab25是小Ras样GTP酶的Rab11家族成员。Rab11（异构体Rab11a和Rab11b）作为核周、细胞膜和高尔基体区域内体再循环的重要调节者而发挥作用（1,2）。尽管有重叠，Rab11a和Rab11b还是在细胞内分布和功能角色中有明确差异。Rab11a广泛表达，Rab11b主要在心脏和脑中发现（3,4）。像其它Rab蛋白一样，Rab11通过与Rab11家族相互作用蛋白（FIPs）相互作用而发挥作用。FIP有三类，但所有都具有一个保守的羧基末端Rab结合域，以允许Rab-FIP蛋白相互作用。当这些蛋白结合在一起时，它们被认为可以调节膜相关的蛋白分选（5,6）。

1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.

原厂资料：

Specificity / Sensitivity

Rab11b Antibody detects endogenous levels of total Rab11b protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Arg184 of human Rab11b protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).

1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody