Synip (C51G6) Rabbit mAb

• 产品编号：CST-2400S      品牌：CST       原厂货号：2400S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Synip (C51G6)兔单克隆抗体识别内源性的Synip总蛋白水平。Synip (C51G6)兔单克隆抗体用与人类Synip蛋白氨基酸序列对应的人工合成肽段免疫兔制成。胰岛素结合并激活其受体，启动信号级联并最终导致Glut4葡萄糖转运体的从细胞内到细胞膜的易位。起始这一途径后将促进脂肪和骨骼肌细胞对葡萄糖的摄取（1）。Synip和Syntaxin4两种蛋白质被认为参与含Glut4囊泡的质膜招募（2,3）。Synip在缺乏胰岛素时与Syntaxin4结合。胰岛素信号通路触发两种蛋白质的分解，并允许Syntaxin4与VAMP2形成复合物，这是Glut4转位到质膜所必不可少的（2-4）。过表达显性失活形式的Synip可防止胰岛素刺激的Glut4易位到细胞膜（3）。因此，Synip与Syntaxin4共同调节Glut4转运到细胞膜。

1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
2. Min, J. et al. (1999) Mol. Cell 3, 751-760.
3. Yamada, E. et al. (2005) J. Cell Biol. 168, 921-928.
4. Foster, L.J. and Klip, A. (2000) Am. J. Physiol. Cell Physiol. 279, C877-C890.

原厂资料：

Specificity / Sensitivity

Synip (C51G6) Rabbit mAb detects endogenous levels of total Synip protein.

Source / Purification

Synip (C51G6) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence of human Synip.

Background

Insulin binds to and activates its receptor and initiates a signaling cascade that eventually induces the translocation of the Glut4 glucose transporter from its intracellular locations to the plasma membrane. Initiating this pathway facilitates glucose uptake in fat and skeletal muscle cells (1). Synip and Syntaxin 4 are two proteins thought to be involved in the recruitment of Glut4-containing vesicles to plasma membrane (2,3). Synip associates with Syntaxin 4 when insulin is absent. Insulin signaling triggers the dissociation of the two proteins and allows Syntaxin 4 to complex with VAMP2, which is essential for Glut4 translocation to plasma membrane (2-4). Overexpression of a dominant-negative form of Synip prevents Glut4 from translocating to plasma membrane in response to insulin stimulation (3). Synip together with Syntaxin 4, therefore, regulates Glut 4 transport to plasma membrane.

1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
2. Min, J. et al. (1999) Mol. Cell 3, 751-760.
3. Yamada, E. et al. (2005) J. Cell Biol. 168, 921-928.
4. Foster, L.J. and Klip, A. (2000) Am. J. Physiol. Cell Physiol. 279, C877-C890.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody