HA-Tag (6E2) Mouse mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-2350S      品牌：CST       原厂货号：2350S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 488 Conjugate)用来检测具有HA表位标签的重组蛋白，此抗体可以识别转染细胞靶蛋白在氨基或羧基末端融合的HA标签 。单克隆抗体是用含有流感血凝素表位(YPYDVPDYA的合成肽段肽段免疫动物生产的，此种抗体 在最佳条件下采用Alexa Fluor ® 488荧光染料标记，F/P为2-6。此种抗体 在最佳条件下采用Alexa Fluor ® 488荧光染料标记，已经过内部测试，可以直接用于HA标签蛋白转染细胞的流式细胞术和免疫荧光分析。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。HA标签式从流感血凝素蛋白的一个表位演化而来的。已经作为常规标签被广泛应用于表达载体中（1）

1. Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.

原厂资料：

Specificity / Sensitivity

HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate) detects proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofuorescent analysis in cells transfected with HA-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).

1. Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.