LEF1 (C12A5) Rabbit mAb

• 产品编号：CST-2230P      品牌：Cell Signaling Technology       原厂货号：2230P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LEF1 (C12A5)Rabbit mAb兔单抗识别内源性的总LEF1 蛋白，它不识别由选择性启动子产生的负显性LEF1。此单抗由重组蛋白免疫动物产生，该重组蛋白与人LEF1邻近Pro82氨基酸残基序列一致。

LEF1和TCF是高迁移率族蛋白(HMG)中DNA结合蛋白家族的成员中的一类转录因子，包括：Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1)。LEF1和TCF1最初被认为是调节早期淋巴发育的重要因子(2)，并作为Wnt的下游信号发挥作用。LEF1和TCF结合Wnt反应元件为β-catenin提供停泊位点，β-catenin随后转移到细胞核促进Wnt信号通路靶基因的转录激活(3)。在包括结肠癌（4，5）在内的多种癌症发生相关的Wnt信号通路的发展和异常活化中，都会伴随着LEF1和TCF表达水平的动态变化。LEF1因选择性剪接而有多种异构体。LEF1也有一个选择性启动子，在淋巴细胞中优先激活，由选择性启动子起始的一种异构体没有氨基末端的β-catenin结合结构域，并可能的功能为负显性调控（6-8）。

1. Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52.
2. Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32.
3. Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
4. Reya, T. and Clevers, H. (2005) Nature 434, 843-50.
5. Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
6. Hovanes, K. et al. (2000) Nucleic Acids Res. 28, 1994-2003.
7. Hovanes, K. et al. (2001) Nat. Genet. 28, 53-57.
8. Kobielak, A. et al. (2001) Acta. Biochim. Pol. 48, 221-226.

原厂资料：

Specificity / Sensitivity

LEF1 (C12A5) Rabbit mAb detects endogenous level of total LEF1 protein. It does not recognize the dominant negative forms of LEF1 generated by an alternative promoter.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro82 of human LEF1.

Background

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).LEF1 has several isoforms due to alternative splicing. LEF1 also has an alternative promoter that is preferentially active in lymphocytes. The isoforms generated by this alternative promoter have no amino-terminal β-catenin binding domain and may function in a dominant negative manner (6-8).

1. Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52.
2. Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32.
3. Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
4. Reya, T. and Clevers, H. (2005) Nature 434, 843-50.
5. Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
6. Hovanes, K. et al. (2000) Nucleic Acids Res. 28, 1994-2003.
7. Hovanes, K. et al. (2001) Nat. Genet. 28, 53-57.
8. Kobielak, A. et al. (2001) Acta. Biochim. Pol. 48, 221-226.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.