ESET (C1C12) Rabbit mAb

• 产品编号：CST-2196S      品牌：CST       原厂货号：2196S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ESET (C1C12) Rabbit mAb兔单抗能够检测内源性ESET总蛋白水平。该抗体不与包含SET区域的组蛋白甲基转移酶发生交叉反应。通过合成的与人源ESET蛋白羧基端相应多肽片段去免疫动物从而制备出此单克隆抗体。

Erg-associated protein with SET domain (ESET)也称为SET-domain bifurcated 1 (SETDB1) protein，它是一个组蛋白赖氨酸甲基转移酶家族成员，它们中每一个都包含一个保守催化的SET结构，该结构起初被鉴定在Drosophila Su[var]3-9、Enhancer of zeste和Trithorax蛋白中(1)。ESET也包含tudor和methyl-CpG-binding区域，这可以协调分别结合到甲基化组蛋白和甲基化的DNA(1)。ESET可使 histone H3蛋白Lys9位点甲基化，这增加一个有助于基因沉默的转录性抑制标记物(1-3)。然而，不像SUV39H histone H3 Lys9 甲基转移酶一样，该酶的功能主要在异染色质区域例如臂间异染色质（pericentric heterochromatin），ESET的功能主要在常染色质区域去抑制基因启动子(3)。ESET与多种蛋白质相互作用，包括转录因子(ERG)、组蛋白去乙酰酶 (HDAC1/2)、DNA甲基转移酶(DNMT3A/B) 和转录辅助抑制因子(mSin3A/B、MBD1、KAP-1、ATFa-associated modulator mAM) (1-6)。mAM形成一个ESET复合物，这能刺激它的甲基转移酶活性，特别是histone H3蛋白Lys9位点双甲基化转化到三甲基化(2)。在S期复制偶联染色质装配期间，MBD1招募ESET到CAF-1复合物从而有助于histone H3蛋白Lys9位点甲基化。在癌症细胞中DNMT3A蛋白招募ESET到沉默的启动子(7)。因为ESET蛋白水平和histone H3蛋白Lys9位点三甲基化都在病理大脑中表达增加，ESET可能在亨廷顿氏舞蹈病的发病机理上有重要作用(8)。

1. Yang, L. et al. (2002) Oncogene 21, 148-152.
2. Wang, H. et al. (2003) Mol. Cell 12, 475-487.
3. Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
4. Yang, L. et al. (2003) Biochem. J. 369, 651-657.
5. Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
6. Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
7. Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
8. Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.

原厂资料：

Specificity / Sensitivity

ESET (C1C12) Rabbit mAb detects endogenous levels of total ESET protein. The antibody does not cross-react with other SET-domain containing histone methyltransferase proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein.

Background

The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).

1. Yang, L. et al. (2002) Oncogene 21, 148-152.
2. Wang, H. et al. (2003) Mol. Cell 12, 475-487.
3. Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
4. Yang, L. et al. (2003) Biochem. J. 369, 651-657.
5. Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
6. Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
7. Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
8. Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.