RPA70/RPA1 (C24F2) Rabbit mAb

• 产品编号：CST-2193S      品牌：CST       原厂货号：2193S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RPA70 (C32F2) Antibody 能够检测内源性RPA70总蛋白的水平。该单克隆抗体是由合成的针对人RPA70的164位苏氨酸残基的肽段免疫动物生产的。

RPA70 (HSSB, REPA1, RF-A, RP-A, p70)是异源三聚体复合物的组成部分，由70、32/30和14 kDa的亚基组成，统称RPA。RPA是一种单链DNA结合蛋白，其DNA结合活性完全位于70 kDa的亚基。几乎是所有的DNA代谢过程如DNA复制(1-3)、重组、细胞周期和DNA损伤检验点以及所有主要类型的DNA修复包括核苷酸切除、碱基切除、错配和双链断裂修复都需要该复合体的参与(4-7)。应答真核细胞基因毒性应激反应的过程中，RPA被认为可以协同Rad9/Rad1/Hus1 (9-1-1)检验点复合体(8)。DNA损伤时或者检验点激酶包括ATM、ATM-Rad3、ATR和DNA-依赖性蛋白激酶(DNA-PK) (9-11)引起的复制应激中，RPA是过度磷酸化的。过度磷酸化可以改变RPA-DNA和RPA-蛋白相互作用。除检验点伴侣之外，RPA能够和多种蛋白伴侣相互作用，包括正常复制所需蛋白如RCF，PCNA和Pol α以及参与SV40复制的蛋白如DNA聚合酶I和SV40大T抗原(10,12)。

1. Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231.
2. Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838.
3. Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218.
4. Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527.
5. Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439.
6. Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323.
7. Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85.
8. Guo, S. et al. (2006) J Biol Chem 281, 21607-16.
9. Wu, X. et al. (2005) Oncogene 24, 4728-4735.
10. Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024.
11. Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437.
12. Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.

原厂资料：

Specificity / Sensitivity

RPA70 (C32F2) Antibody detects endogenous levels of total RPA70 protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptide corresponding to amino acids surrounding threonine 164 of human RPA70.

Background

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).

1. Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231.
2. Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838.
3. Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218.
4. Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527.
5. Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439.
6. Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323.
7. Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85.
8. Guo, S. et al. (2006) J Biol Chem 281, 21607-16.
9. Wu, X. et al. (2005) Oncogene 24, 4728-4735.
10. Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024.
11. Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437.
12. Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody