Alix (3A9) Mouse mAb

• 产品编号：CST-2171S      品牌：CST       原厂货号：2171S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Alix (3A9) Mouse mAb 鼠单抗能够检测内源Alix总蛋白。该单克隆抗体是采用人源全长Alix蛋白来免疫动物而获得。

Alix是系统发育中保守的细胞质支架蛋白, 包含一个N-末端的Bro1域, 一个卷曲螺旋域和一个C-末端富含脯氨酸域(1,2)。最初鉴定其为ALG-2 (凋亡连接基因2)互做蛋白，参与到程序性细胞死亡过程中(3,4), Alix也能调控很多其他的细胞过程，如内吞膜的转运，与 ESCRT (转运所需的胞内分选复合体)、endophilins和CIN85 (Cbl-互作85 kDa蛋白)蛋白相互作用形成的细胞粘连(5,6)。

1. Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
2. Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
3. Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
4. Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
5. Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
6. Sadoul, R. (2006) Biol. Cell 98, 69-77.

原厂资料：

Specificity / Sensitivity

Alix (3A9) Mouse mAb detects endogenous levels of total Alix protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein.

Background

Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).

1. Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
2. Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
3. Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
4. Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
5. Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
6. Sadoul, R. (2006) Biol. Cell 98, 69-77.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.