α-N-Catenin Antibody

• 产品编号：CST-2131S      品牌：CST       原厂货号：2131S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

α-N-Catenin抗体可以识别内源性α-N-Catenin蛋白。该抗体与其他α-Catenin家族成员没有交叉反应。合成对应人α-N-Catenin羧基端的多肽序列免疫动物获得多克隆抗体。抗体经过蛋白A和肽亲和层析纯化。

细胞黏附是形成细胞间联系的动态结构，在发育，分化，组织整合，形态发生和细胞极化过程中都非常重要。它们由跨膜蛋白和钙粘附蛋白组成，钙粘附蛋白与邻近细胞在钙依赖的模式下互相结合。在黏附连接的细胞质部分，经典状态下钙粘附蛋白会通过β- 和α-catenin与细胞骨架连接。α-E-catenin广泛表达，α-N-catenin在神经组织中表达，α-T-catenin最初表达于心脏组织。在某些人类癌症进程中E-cadherin和α-E-catenin会发生缺失，表明黏附连接的破坏对于癌症进展是重要的（reviewed in 1）。最近的证据表明，与其作为钙粘附蛋白和肌动蛋白间稳定的连接，α-catenin直接调控肌动蛋白的动态过程，很可能是通过与肌动蛋白核化arp2/3复合体竞争而进行的（2,3）。α-catenin也在调控β-catenin依赖的转录过程中发挥作用，影响分化，并反馈Wnt信号。α-catenin在细胞核内与β-catenin结合，阻止其发挥转录作用，两种蛋白的表达水平似乎都是经过蛋白酶体依赖的降解而受到调控的（4）。

1. Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
2. Yamada, S. et al. (2005) Cell 123, 889-901.
3. Drees, F. et al. (2005) Cell 123, 903-15.
4. Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.

原厂资料：

Specificity / Sensitivity

α-N-Catenin Antibody detects endogenous levels of α-N-catenin protein. The antibody does not cross-react with other α-catenin family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding the the carboxy-terminus of human α-N-catenin. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). 
 Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).

1. Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
2. Yamada, S. et al. (2005) Cell 123, 889-901.
3. Drees, F. et al. (2005) Cell 123, 903-15.
4. Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.