GAPDH (14C10) Rabbit mAb

• 产品编号：CST-2118S      品牌：CST       原厂货号：2118S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GAPDH (14C10)兔单克隆抗体识别内源性的GAPDH总蛋白水平。该单克隆抗体用与人类GAPDH中羧基末端对应的人工合成肽段免疫动物而制成。

甘油醛-3-磷酸脱氢酶（GAPDH）催化糖酵解过程中的甘油醛-3-磷酸的磷酸化。虽然在组织之间表达有差异（1），但GAPDH被认为是作为持家基因组成性表达的。出于这个原因，GAPDH的mRNA和蛋白水平往往是作为对照，量化的其他目标的基因或蛋白的表达变化。 最近的工作阐明了GAPDH在细胞凋亡（2），基因表达（3）和核运输（4）中的角色。 GAPDH在神经退行性病变，如亨廷顿和阿尔茨海默氏病（4,5）中也可起到一定的作用。

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody