SRC-3 (11B1) Mouse mAb

• 产品编号：CST-2115S      品牌：CST       原厂货号：2115S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SRC-3 (11B1) Mouse mAb鼠单抗能够检测内源性SRC-3总蛋白水平。通过重组人源SRC-1多肽片段(a.a. 1-250)去免疫动物从而制备出此单克隆抗体。

steroid receptor co-activator (SRC) 家族有三个成员蛋白：RC-1 (NCoA-1)、SRC-2 (TIF2/GRIP1/NCoA-2)和SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1)。所有SRC家族蛋白都有共同的结构同源性和功能，该功能是通过核激素受体和其他转录激活因子例如STAT3、NF-κB、E2F1和p53介导一个类似的方式去刺激转录（1-4）。两个SRC蛋白SRC-1和SRC-3都具有组蛋白乙酰转移酶的功能（5,6）。此外，所有的三个家族的蛋白都可以招募其他的组蛋白乙酰转移酶(CBP/p300, PCAF) 和组蛋白甲基转移酶(PRMT1, CARM1) 去定位到启动子并协助其增强许多基因的转录（5-8）。SRC蛋白在许多生理过程包括：细胞增殖，细胞存活，体细胞生长， 乳腺发育，雌性生殖功能以及血管保护中都起着重要作用（9）。SRC-1和SRC-3是激酶介导的生长因子信号传导到雌激素受体和其他转录激活因子的通道。7个SRC-1和6个SRC-3磷酸化位点已经被鉴定，这些位点的磷酸化由类固醇、细胞因子和生长因子所诱导，并且涉及到多个信号通路（9-11）。所有的3个SRC家族成员都与乳腺、前列腺和卵巢肿瘤核内受体的活性增加有关。此外，在多种癌症中SRC-3都频繁放大或过量表达（12），并且SRC-1/PAX3和SRC-2/MYST3的翻译分别与横纹肌肉瘤和急性髓细胞白血病有关联（13,14）。

1. Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
2. Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
3. Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
4. Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
5. Spencer, T.E. et al. (1997) Nature 389, 194-198.
6. Chen, H. et al. (1997) Cell 90, 569-580.
7. Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
8. Chen, D. et al. (1999) Science 284, 2174-2177.
9. Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
10. Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
11. Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
12. Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
13. Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
14. Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.

原厂资料：

Specificity / Sensitivity

SRC-3 (11B1) Mouse mAb detects endogenous levels of total SRC-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human SRC-3 polypeptide fragment (a.a. 1-250).

Background

There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2) and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). The SRC family members all share significant structural homology and function in a similar fashion to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as STAT3, NF-κB, E2F1 and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines and growth factors and involve multiple kinase signaling pathways (9-11). All three SRC family members are associated with increased activity of nuclear receptors in breast, prostate and ovarian carcinomas. In addition, SRC-3 is frequently amplified or over-expressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).

1. Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
2. Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
3. Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
4. Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
5. Spencer, T.E. et al. (1997) Nature 389, 194-198.
6. Chen, H. et al. (1997) Cell 90, 569-580.
7. Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
8. Chen, D. et al. (1999) Science 284, 2174-2177.
9. Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
10. Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
11. Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
12. Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
13. Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
14. Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody