Rictor (53A2) Rabbit mAb

• 产品编号：CST-2114S      品牌：CST       原厂货号：2114S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rictor (53A2) Rabbit mAb兔单抗能够检测内源性的rictor总蛋白。Rictor (53A2) Rabbit mAb兔单抗是由合成的针对人rictor蛋白Gln1681位点周围残基的肽段免疫兔而生产的。

细胞生长是一个基本的生物学过程，细胞积累物质并增加体积。哺乳动物TOR（mTOR）信号通路通过调节生长因子驱动的信号和能量及营养信号调控细胞生长（1）。mTOR是一个有两种不同形式复合物的蛋白激酶。一个复合物包含mTOR，GβL和raptor，是rapamycin的靶点。另一个复合物包括mTOR，GβL，SIN1和rictor，对rapamycin不敏感（1）。mTOR-rictor复合物体外磷酸化修饰Akt/PKB的Ser473位点（2）。磷酸化后可充分激活Akt / PKB。此外，在3T3-L1脂肪细胞里siRNA降低rictor的表达可以抑制Ser473位点磷酸化（3）。这个复合物也可以磷酸化抗rapamycin的S6K1突变体，另一个mTOR效应分子（4）。

1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.

原厂资料：

Specificity / Sensitivity

Rictor (53A2) Rabbit mAb detects endogenous levels of total Rictor protein.

Source / Purification

Rictor (53A2) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence Gln1681 of human Rictor.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.