Grp94 Antibody

• 产品编号：CST-2104P      品牌：Cell Signaling Technology       原厂货号：2104P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Grp94 Antibody识别内源性的Grp94总蛋白。该多克隆抗体用与人类Grp94蛋白Met622附近氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

分泌型和跨膜型蛋白以多核糖体形式合成，然后转移到内质网中。在内质网中，这些蛋白进行常见的二硫键形成、氨基糖基化和折叠。内质网也包含一组分子伴侣，包括Grp94，以帮助蛋白质正确折叠。Grp94是葡萄糖调节蛋白（1），与Hsp90有序列同源性（2）。除了能帮助分泌蛋白质折叠到它们正确的构想（3），研究显示癌细胞中产生的Grp94也能诱导鼠模型中的抗肿瘤免疫反应（4，5）。此肿瘤免疫原性因为Grp94可以结合到癌细胞的蛋白质的多肽上，然后将其呈示为肿瘤抗原（6）。此外，Grp94也能够诱导树突细胞成熟（7）。综上，Grp94既可以是肿瘤特异的抗原，也可以作为抗原呈递细胞对癌细胞免疫性的激活者（8）。

1. Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925.
2. Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344.
3. Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505.
4. Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322.
5. Tamura, Y. et al. (1997) Science 278, 117-120.
6. Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101.
7. Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215.
8. Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331.

原厂资料：

Specificity / Sensitivity

Grp94 Antibody detects endogenous levels of total Grp94 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Met622 of human Grp94 . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role to help some secretory proteins fold to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One reason for this tumor immunogenicity is that Grp94 binds to the peptides from proteins in cancer cells and can therefore present these peptides as tumor antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit immunity to cancers (8).

1. Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925.
2. Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344.
3. Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505.
4. Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322.
5. Tamura, Y. et al. (1997) Science 278, 117-120.
6. Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101.
7. Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215.
8. Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody