RhoB Antibody

• 产品编号：CST-2098P      品牌：Cell Signaling Technology       原厂货号：2098P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RhoB Antibody检测内源性RhoB总蛋白。该抗体不能识别相关蛋白RhoA或RhoC蛋白。通过人工合成人源RhoB蛋白羧基端周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Rho family small GTPases包括Rho、Rac和cdc42，该家族作为分子开关，调节细胞进程例如细胞迁移、粘附、扩增和分化。它们通过鸟苷酸交换因子（guanine nucleotide exchange factor，GEF)被激活，这将催化已结合的GDP交换成GTP，然而通过GTPase activating proteins (GAPs)被抑制，这将催化GTP的水解成GDP。第三个调节水平通过Rho GDP dissociation inhibitor (RhoGDI)的计量结合力被提供(1)。RhoA、RhoB和RhoC是高度保守的，但是出现不同的生物功能。羧基端的修饰和在亚细胞上的定位差异允许这些蛋白回应和作用于不同的信号分子(2,3)。

RhoB蛋白功能是调节细胞形态、迁移和粘附(4)。RhoB蛋白活性也已经显示在蛋白质运输(5,6)和CXCR2介导的趋化性(6)中起着一定作用。PKC激酶的RhoB活性下游分子的抑制影响恶性胶质瘤细胞的入侵程度和迁移(7)，并且RhoB 蛋白表达在卵巢癌的肿瘤生长中有一个负性影响(8)。

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
4. Wheeler, A.P. and Ridley, A.J. (2007) Exp Cell Res 313, 3505-16.
5. Sandilands, E. et al. (2007) J Cell Sci 120, 2555-64.
6. Neel, N.F. et al. (2007) J Cell Sci 120, 1559-71.
7. Baldwin, R.M. et al. (2008) Oncogene 27, 3587-95.
8. Couderc, B. et al. (2008) Cancer Gene Ther 15, 456-64.

原厂资料：

Specificity / Sensitivity

RhoB Antibody detects endogenous levels of total RhoB protein. The antibody does not cross-react with RhoA or RhoC.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RhoB. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).RhoB functions in the regulation of cell shape, migration and adhesion (4). RhoB activity has also been shown to play a role in protein trafficking (5,6) and in CXCR2-mediated chemotaxis (6). Inhibition of RhoB activity downstream of PKCι influences the degree of invasion and migration by glioblastoma cells (7), and RhoB expression has a negative affect on tumor growth in ovarian cancer (8).

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
4. Wheeler, A.P. and Ridley, A.J. (2007) Exp Cell Res 313, 3505-16.
5. Sandilands, E. et al. (2007) J Cell Sci 120, 2555-64.
6. Neel, N.F. et al. (2007) J Cell Sci 120, 1559-71.
7. Baldwin, R.M. et al. (2008) Oncogene 27, 3587-95.
8. Couderc, B. et al. (2008) Cancer Gene Ther 15, 456-64.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.