Axin1 (C95H11) Rabbit mAb

• 产品编号：CST-2074S      品牌：CST       原厂货号：2074S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Axin1 (C95H11)Rabbit mAb兔单抗识别内源性的总Axin1 蛋白.此单抗由重组人源Axin1蛋白免疫动物产生.

Axin1（轴心抑制蛋白1）和Axin2为多结构域支架蛋白，并负向调控Wnt信号通路。Axin1与APC, GSK-3β, Dvl和 β-catenin相互作用促进GSK-3β调控的磷酸化并随后β-catenin的降解（1,2）。由于Wnt引起的细胞刺激，Axin1被磷酸化的LRP 5/6招募到细胞膜上,这个过程被认为是Wnt信号通路激活的关键（3,4）。除了在Wnt信号上的作用外，Axin1和MEKK1形成复合物，并激活c-jun氨基末端激酶（JNK/SAPK）（5）。Axin2(也称Conductin 或Axil)在小鼠体内功能上能代替Axin1（6）。Axin2自身直接是Wnt信号通路的靶标并因此通过负向反馈机制参与控制Wnt通路的强度和持续时间（7-9）。

1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
3. Zeng, X. et al. (2005) Nature 438, 873-877.
4. Davidson, G. et al. (2005) Nature 438, 867-872.
5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

原厂资料：

Specificity / Sensitivity

Axin1 (C95H11) Rabbit mAb detects the endogenous levels of total Axin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein of human Axin1.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
3. Zeng, X. et al. (2005) Nature 438, 873-877.
4. Davidson, G. et al. (2005) Nature 438, 867-872.
5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody