Mili Antibody

• 产品编号：CST-2071S      品牌：CST       原厂货号：2071S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mili Antibody检测内源性Mili总蛋白水平。通过人工合成小鼠Mili蛋白相应序列的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

果蝇piwi 基因被鉴定是生殖系干细胞自我更新所必须的(1)。在不同的物种包括拟南芥、秀丽隐杆线虫和人类中，Piwi同源物都很保守(1)。Miwi和Mili蛋白都是小鼠Piwi 同源物，且都包含有 C末端Piwi结构域(2)。Miwi和Mili蛋白结合到在雄性生殖细胞中Piwi-interacting RNAs (piRNAs)，并且对小鼠精子的生成是至关重要的(3-5)。

1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
3. Aravin, A. et al. (2006) Nature 442, 203-7.
4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.

原厂资料：

Specificity / Sensitivity

Mili Antibody detects endogenous levels of total Mili protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse Mili. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Drosophila piwi gene was identified as being required for the self-renewal of germ-line stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and human (1). Miwi and Mili proteins are both mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (3-5).

1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
3. Aravin, A. et al. (2006) Nature 442, 203-7.
4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody