GFP (D5.1) XP? Rabbit mAb (HRP Conjugate)

• 产品编号：CST-2037S      品牌：CST       原厂货号：2037S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

GFP (D5.1) XP ® Rabbit mAb（HRP 标记）用来检测外源性表达在细胞中GFP标签的蛋白。单克隆抗体是采用GFP氨基末端相对应的合成肽段免疫动物生产的，此抗体在最佳条件下结合了HRP。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。

绿色荧光蛋白（GFP）在水母作为生物发光能量转移受体，其最大吸收光波长为395nm，发射谱的最大峰值为509nm。GFP作为一种融合蛋白已经成为报告基因表达，追踪细胞系和蛋白亚细胞定位的重要工具（1）。

1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

原厂资料：

Specificity / Sensitivity

GFP (D5.1) XP® Rabbit mAb (HRP Conjugate) detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. This antibody was conjugated to HRP under optimal conditions.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).

1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic and 50% stabilizer.
Store at 4ºC.